Researchers have examined a number of combos of elements with anti-aging properties. These combos, together with a 12-ingredient formulation created by NOVOS, helped to reduce DNA damage and oxidative stress in human skin cells in cultures [1].
Combating a number of Hallmarks of Getting old directly
Getting old impacts a number of mobile processes, and these impacts could be assessed via biomarkers. The researchers of this paper used human pores and skin cells (keratinocytes) as their targets for assessing the influence of the NOVOS complement and 6 different formulations on DNA injury and oxidative stress markers. They consider that since pores and skin is ceaselessly uncovered to DNA-damaging brokers, equivalent to UV mild, it’s the proper selection for assessing these markers.
The examined complement formulation created by NOVOS, a New York-based firm, is a mixture of 12 elements: pterostilbene, a resveratrol-related polyphenol derived from blueberries; glucosamine sulfate, a complement used for osteoarthritis therapy; fisetin, a senotherapeutic; glycine, an amino acid; lithium aspartate, a mineral; calcium alpha-ketoglutarate, a small molecule current within the human physique; magnesium malate, a pure substance present in apples; vitamin C, also called ascorbic acid; L-theanine, an amino acid discovered primarily in inexperienced and black tea; hyaluronic acid; Rhodiola rosea root extract; ginger root extract; and a lift consisting of β-nicotinamide mononucleotide.
These nature-based elements, all of which have good security profiles, had been chosen based mostly on their anti-aging exercise, documented function in counteracting a number of getting older processes, or affiliation with aging-related mechanisms. We now have previously written about them and their choice course of in additional element.
The authors defined that they examined combos of various compounds as a result of this method may deal with a number of getting older processes directly. A mix of compounds may additionally successfully cut back undesirable unwanted effects that may seem when a single compound is utilized in excessive concentrations.
Discount in DNA injury
Even helpful elements could be poisonous in extreme concentrations. Due to this fact, the researchers began their research by assessing the compounds’ highest secure concentrations to make use of. They adopted up by testing the influence of complement formulation on defending cells from DNA injury, utilizing tumor p53 binding protein 1 (53BP1) as a DNA injury marker.
The researchers handled 2D cell cultures with the six formulations and NOVOS. Following this therapy, they induced DNA injury with the genotoxic agent neocarzinostatin (NCS).
Knowledge evaluation revealed that treating cells with the six totally different formulations induced delicate opposed results on cells, as evidenced by larger numbers of DNA damage-related foci in some cells. NOVOS therapy didn’t trigger that.
Cells pre-treated with NOVOS had been much less prone to have many of those foci after NCS administration, which means that NOVOS helps alleviate the DNA injury attributable to NCS. Nonetheless, it didn’t carry the injury right down to the extent of management cells that weren’t uncovered to NCS.
The six totally different formulations additionally positively affected cells uncovered to NCS. These formulation “considerably prevented DNA injury, noticed as decreased foci numbers, even with a stronger impact in comparison with the NOVOS’ unique formulation itself.”
Primarily based on this statistical evaluation and the variety of elements in every formulation, the researchers selected NOVOS, F1, F2, and F4 for the next experiments.
The primary was a take a look at of another DNA injury marker, γ-H2AX, a marker that displays double-strand breaks within the DNA. Nonetheless, they carried out it within the 3D pores and skin cell cultures since 3D cultures higher replicate the pores and skin microenvironment and ”permit the formation of a mobile construction, like a tissue.” The outcomes of this experiment steered that each one 4 of those formulations considerably decreased DNA injury in comparison with cells that had been solely uncovered to NCS.
Notably, the focus of NOVOS used on this experiment is decrease than that of different formulations. Nonetheless, it might obtain comparable results in counteracting DNA injury as F1, F2, and F4. The authors counsel that this can be due to some synergistic results between the NOVOS compounds.
Decreased oxidative stress
Within the following take a look at, the researchers aimed to evaluate the formulations’ means to forestall oxidative injury to the cells. Intracellular reactive oxygen species (ROS) are tough to measure. Nonetheless, since a lot of them are transformed to hydrogen peroxide, hydrogen peroxide can be utilized as a proxy for intracellular ROS stage adjustments and oxidative stress.
The researchers carried out this experiment utilizing 2D and 3D cultures. These cells had been handled with totally different F1, F2, F4, and NOVOS formulation concentrations. Then, the researchers used the pro-oxidant medicine to induce oxidative injury.
F1 and F4 formulations had been capable of considerably cut back intracellular hydrogen peroxide manufacturing following menadione therapy in 2D and 3D cell cultures. NOVOS was capable of obtain this in 2D however not 3D tradition.
Limitations
This research means that NOVOS, together with the F1, F2, and F4 combos, are capable of counteract some aging-related processes in pores and skin cells.
Whereas the researchers mentioned the advantages of utilizing a mix of compounds over a single compound, this analysis lacks an experiment that compares the combos to single compounds. Such experiments could be very informative in testing whether or not the noticed results outcome from the mixed impact of the a number of compounds or whether or not the noticed results come primarily from the motion of a single compound and there’s a negligible contribution from the remaining compounds, which might permit for easier dietary supplements. That is notable because the F1, F2, and F4 combos with half the NOVOS compounds present comparable results as NOVOS within the experiments.
The authors additionally notice that additional investigation into particular molecular pathways concerned within the noticed processes is important.
One of many research authors is an worker of NOVOS Labs. Nonetheless, within the Battle of Curiosity, the authors notice: ”The funder NOVOS Labs was not concerned within the research design, assortment, evaluation, interpretation of information, of this text or the choice to submit it for publication.”
Disclosure: A portion of the income and fairness from NOVOS are being donated to nonprofits working within the longevity science area, which incorporates us right here at Lifespan.io.
Our Vice President, Dr. Oliver Medvedik, can also be a scientific marketing consultant, placing him in good firm with Dr. Joao Pedro Magalhaes, Dr. Pamela Maher, Dr. Avi Rosenbaum, and Dr. Matt Kaeberlein, names with whom common readers could also be acquainted.
Literature
[1] Punzo, A., Perillo, M., Silla, A., Malaguti, M., Hrelia, S., Diogo Barardo, Cristiana Caliceti, & Lorenzini, A. (2024). Promising Results of Novel Complement Formulation in Stopping Pores and skin Getting old in 3D Human Keratinocytes. Vitamins, 16(16), 2770–2770.
